Compare Model Drawings, CAD & Specs Availability Price
fluorescence imaging optical filter set
HPF1200
Fluorescence Optical Filter Set, Hoechst 33342 and 33258 Flourophores
$775
In Stock
 HPF1200 Fluorescence Optical Filter Set, Hoechst 33342 and 33258 Flourophores
In Stock
 $775
fluorescence imaging optical filter set
HPF1205
Fluorescence Optical Filter Set, Alexa Fluor 350, AMCA, DAPI
$775
In Stock
 HPF1205 Fluorescence Optical Filter Set, Alexa Fluor 350, AMCA, DAPI
In Stock
 $775
fluorescence imaging optical filter set
HPF1210
Fluorescence Optical Filter Set, LysoSensor™ Blue (pH 5)
$775
In Stock
 HPF1210 Fluorescence Optical Filter Set, LysoSensor™ Blue (pH 5)
In Stock
 $775
fluorescence imaging optical filter set
HPF1215
Fluorescence Optical Filter Set, Pacific Blue™
$775
2 Weeks
 HPF1215 Fluorescence Optical Filter Set, Pacific Blue™
2 Weeks
 $775
fluorescence imaging optical filter set
HPF1220
Fluorescence Optical Filter Set, CFP
$775
2 Weeks
 HPF1220 Fluorescence Optical Filter Set, CFP
2 Weeks
 $775
fluorescence imaging optical filter set
HPF1225
Fluorescence Optical Filter Set, GFP (wt)
$775
In Stock
 HPF1225 Fluorescence Optical Filter Set, GFP (wt)
In Stock
 $775
fluorescence imaging optical filter set
HPF1230
Fluorescence Optical Filter Set, 6-JOE
$775
In Stock
 HPF1230 Fluorescence Optical Filter Set, 6-JOE
In Stock
 $775
fluorescence imaging optical filter set
HPF1235
Fluorescence Optical Filter Set, Rhodamine Green , YGFP
$775
In Stock
 HPF1235 Fluorescence Optical Filter Set, Rhodamine Green , YGFP
In Stock
 $775
fluorescence imaging optical filter set
HPF1240
Fluorescence Optical Filter Set, GFP
$775
In Stock
 HPF1240 Fluorescence Optical Filter Set, GFP
In Stock
 $775
HPF1245
Fluorescence Optical Filter Set, 5-FAM, Alexa Fluor 488
$775
2 Weeks
 HPF1245 Fluorescence Optical Filter Set, 5-FAM, Alexa Fluor 488
2 Weeks
 $775
fluorescence imaging optical filter set
HPF1250
Fluorescence Optical Filter Set, BODIPY FL, DiO, FITC, Fluo 3, Fluo 4, GFP(rs)
$775
In Stock
 HPF1250 Fluorescence Optical Filter Set, BODIPY FL, DiO, FITC, Fluo 3, Fluo 4, GFP(rs)
In Stock
 $775
fluorescence imaging optical filter set
HPF1255
Fluorescence Optical Filter Set, Alexa Fluor 430
$775
In Stock
 HPF1255 Fluorescence Optical Filter Set, Alexa Fluor 430
In Stock
 $775
fluorescence imaging optical filter set
HPF1260
Fluorescence Optical Filter Set, Oregon Green™
$775
In Stock
 HPF1260 Fluorescence Optical Filter Set, Oregon Green™
In Stock
 $775
fluorescence imaging optical filter set
HPF1265
Fluorescence Optical Filter Set, YFP
$775
In Stock
 HPF1265 Fluorescence Optical Filter Set, YFP
In Stock
 $775
fluorescence imaging optical filter set
HPF1270
Fluorescence Optical Filter Set, Eosin
$775
2 Weeks
 HPF1270 Fluorescence Optical Filter Set, Eosin
2 Weeks
 $775
fluorescence imaging optical filter set
HPF1275
Fluorescence Optical Filter Set, Alexa Fluor 532
$775
In Stock
 HPF1275 Fluorescence Optical Filter Set, Alexa Fluor 532
In Stock
 $775
fluorescence imaging optical filter set
HPF1280
Fluorescence Optical Filter Set, 6-TET
$775
In Stock
 HPF1280 Fluorescence Optical Filter Set, 6-TET
In Stock
 $775
fluorescence imaging optical filter set
HPF1285
Fluorescence Optical Filter Set, BODIPY R6G
$775
In Stock
 HPF1285 Fluorescence Optical Filter Set, BODIPY R6G
In Stock
 $775
fluorescence imaging optical filter set
HPF1290
Fluorescence Optical Filter Set, PE (B,R)
$775
2 Weeks
 HPF1290 Fluorescence Optical Filter Set, PE (B,R)
2 Weeks
 $775
fluorescence imaging optical filter set
HPF1295
Fluorescence Optical Filter Set, 6-HEX
$775
In Stock
 HPF1295 Fluorescence Optical Filter Set, 6-HEX
In Stock
 $775
fluorescence imaging optical filter set
HPF1300
Fluorescence Optical Filter Set, Alexa Fluor 546, Alexa Fluor 555, TRITC
$775
In Stock
 HPF1300 Fluorescence Optical Filter Set, Alexa Fluor 546, Alexa Fluor 555, TRITC
In Stock
 $775
fluorescence imaging optical filter set
HPF1305
Fluorescence Optical Filter Set, BODIPY TMR
$775
In Stock
 HPF1305 Fluorescence Optical Filter Set, BODIPY TMR
In Stock
 $775
fluorescence imaging optical filter set
HPF1310
Fluorescence Optical Filter Set, Cy3™
$775
In Stock
 HPF1310 Fluorescence Optical Filter Set, Cy3™
In Stock
 $775
fluorescence imaging optical filter set
HPF1315
Fluorescence Optical Filter Set, Dil(rs)
$775
In Stock
 HPF1315 Fluorescence Optical Filter Set, Dil(rs)
In Stock
 $775
fluorescence imaging optical filter set
HPF1320
Fluorescence Optical Filter Set, DsRed
$775
In Stock
 HPF1320 Fluorescence Optical Filter Set, DsRed
In Stock
 $775
fluorescence imaging optical filter set
HPF1325
Fluorescence Optical Filter Set, Cy3.5™
$775
In Stock
 HPF1325 Fluorescence Optical Filter Set, Cy3.5™
In Stock
 $775
fluorescence imaging optical filter set
HPF1330
Fluorescence Optical Filter Set, Rhodamine
$775
In Stock
 HPF1330 Fluorescence Optical Filter Set, Rhodamine
In Stock
 $775
fluorescence imaging optical filter set
HPF1335
Fluorescence Optical Filter Set, 5-TAMRA, MitoTracker Orange
$775
In Stock
 HPF1335 Fluorescence Optical Filter Set, 5-TAMRA, MitoTracker Orange
In Stock
 $775
fluorescence imaging optical filter set
HPF1340
Fluorescence Optical Filter Set, 5-ROX, MitoTracker Red
$775
In Stock
 HPF1340 Fluorescence Optical Filter Set, 5-ROX, MitoTracker Red
In Stock
 $775
fluorescence imaging optical filter set
HPF1345
Fluorescence Optical Filter Set, Alexa Fluor 568, Alexa Fluor 594
$775
In Stock
 HPF1345 Fluorescence Optical Filter Set, Alexa Fluor 568, Alexa Fluor 594
In Stock
 $775
fluorescence imaging optical filter set
HPF1350
Fluorescence Optical Filter Set, Ethidium Bromide
$775
In Stock
 HPF1350 Fluorescence Optical Filter Set, Ethidium Bromide
In Stock
 $775
fluorescence imaging optical filter set
HPF1355
Fluorescence Optical Filter Set, BODIPY TR
$775
In Stock
 HPF1355 Fluorescence Optical Filter Set, BODIPY TR
In Stock
 $775
fluorescence imaging optical filter set
HPF1360
Fluorescence Optical Filter Set, Nile Red
$775
In Stock
 HPF1360 Fluorescence Optical Filter Set, Nile Red
In Stock
 $775
fluorescence imaging optical filter set
HPF1365
Fluorescence Optical Filter Set, Calcium Crimson
$775
In Stock
 HPF1365 Fluorescence Optical Filter Set, Calcium Crimson
In Stock
 $775
fluorescence imaging optical filter set
HPF1370
Fluorescence Optical Filter Set, Propidium Iodide
$775
2 Weeks
 HPF1370 Fluorescence Optical Filter Set, Propidium Iodide
2 Weeks
 $775
fluorescence imaging optical filter set
HPF1375
Fluorescence Optical Filter Set, Resorufin, Texas Red
$775
2 Weeks
 HPF1375 Fluorescence Optical Filter Set, Resorufin, Texas Red
2 Weeks
 $775
fluorescence imaging optical filter set
HPF1380
Fluorescence Optical Filter Set, BODIPY 630/650
$775
In Stock
 HPF1380 Fluorescence Optical Filter Set, BODIPY 630/650
In Stock
 $775
fluorescence imaging optical filter set
HPF1385
Fluorescence Optical Filter Set, Alexa Fluor 633
$775
In Stock
 HPF1385 Fluorescence Optical Filter Set, Alexa Fluor 633
In Stock
 $775
fluorescence imaging optical filter set
HPF1390
Fluorescence Optical Filter Set, Cy5™
$775
In Stock
 HPF1390 Fluorescence Optical Filter Set, Cy5™
In Stock
 $775
fluorescence imaging optical filter set
HPF1395
Fluorescence Optical Filter Set, BODIPY 650/665
$775
In Stock
 HPF1395 Fluorescence Optical Filter Set, BODIPY 650/665
In Stock
 $775
fluorescence imaging optical filter set
HPF1400
Fluorescence Optical Filter Set, Alexa Fluor 647
$775
In Stock
 HPF1400 Fluorescence Optical Filter Set, Alexa Fluor 647
In Stock
 $775
fluorescence imaging optical filter set
HPF1405
Fluorescence Optical Filter Set, Cy5.5™
$775
In Stock
 HPF1405 Fluorescence Optical Filter Set, Cy5.5™
In Stock
 $775
fluorescence imaging optical filter set
HPF1410
Fluorescence Optical Filter Set, Alexa Fluor 660
$775
In Stock
 HPF1410 Fluorescence Optical Filter Set, Alexa Fluor 660
In Stock
 $775
fluorescence imaging optical filter set
HPF1415
Fluorescence Optical Filter Set, Alexa Fluor 680
$775
In Stock
 HPF1415 Fluorescence Optical Filter Set, Alexa Fluor 680
In Stock
 $775
fluorescence imaging optical filter set
HPF1420
Fluorescence Optical Filter Set, Alexa Fluor 700
$775
In Stock
 HPF1420 Fluorescence Optical Filter Set, Alexa Fluor 700
In Stock
 $775
fluorescence imaging optical filter set
HPF1425
Fluorescence Optical Filter Set, Cy7™
$775
In Stock
 HPF1425 Fluorescence Optical Filter Set, Cy7™
In Stock
 $775
fluorescence imaging optical filter set
HPF1430
Fluorescence Optical Filter Set, Alexa Fluor 750
$775
In Stock
 HPF1430 Fluorescence Optical Filter Set, Alexa Fluor 750
In Stock
 $775

Features

Filter Sets for Fluorescence Microscopy

HPF™ filter sets for fluorescence detection are a compilation of filter designs developed to be used with many of the most popular standard fluorophores and some of the most promising new fluorophores. These product designs have served as the starting point for custom designed filters for market leading instruments including DNA Sequencers, Cytometers, MicroPlate Readers, In vivo Imagers, and Thermocyclers. HPF™ filters are manufactured using our patented Stabilife® coating technology which provides excellent spectral performance, spectral stability, and physical durability.

HPF™ Fluorophore Filter Reference

Fluorophore FilterSet Excitation Filter Dichroic Filter Emission Filter
5-FAM HPF1245 HPX475-44 HPD505 HPM535-50
5-ROX HPF1340 HPX560-50 HPD590 HPM640-60
5-TAMRA HPF1335 HPX525-45 HPD560 HPM620-75
6-HEX HPF1295 HPX520-40 HPD550 HPM590-55
6-JOE HPF1230 HPX500-20 HPD515 HPM530-LP
6-TET HPF1280 HPX500-40 HPD530 HPM575-50
Alexa Fluor 350 HPF1205 HPX360-60 HPD400 HPM460-60
Alexa Fluor 430 HPF1255 HPX425-40 HPD490 HPM540-40
Alexa Fluor 488 HPF1245 HPX475-44 HPD505 HPM535-50
Alexa Fluor 532 HPF1275 HPX500-40 HPD530 HPM560-50
Alexa Fluor 546 HPF1300 HPX525-45 HPD560 HPM600-60
Alexa Fluor 555 HPF1300 HPX525-45 HPD560 HPM600-60
Alexa Fluor 568 HPF1345 HPX560-40 HPD595 HPM640-60
Alexa Fluor 594 HPF1345 HPX560-40 HPD595 HPM640-60
Alexa Fluor 633 HPF1385 HPX610-60 HPD645 HHPM685-50
Alexa Fluor 647 HPF1400 HPX620-50 HPD660 HPM700-60
Alexa Fluor 660 HPF1410 HPX630-60 HPD680 HPM720-60
Alexa Fluor 680 HPF1415 HPX660-40 HPD695 HPM725-40
Alexa Fluor 700 HPF1420 HPX660-40 HPD695 HPM740-50
Alexa Fluor 750 HPF1430 HPX710-50 HPD760 HPM810-80
AMCA HPF1205 HPX360-60 HPD400 HPM460-60
BODIPY 630/650 HPF1380 HPX605-40 HPD635 HPM670-50
BODIPY 650/665 HPF1395 HPX630-40 HPD660 HPM695-50
BODIPY FL HPF1250 HPX560-50 HPD590 HPM535-50
BODIPY R6G HPF1285 HPX510-40 HPD540 HPM575-50
BODIPY TMR HPF1305 HPX530-40 HPD560 HPM600-60
BODIPY TR HPF1355 HPX570-40 HPD610 HPM645-50
Calcium Crimson HPF1365 HPX570-40 HPD595 HPM645-60
CFP HPF1220 HPX440-20 HPD455 HPM480-40
Cy3.5™ HPF1325 HPX565-30 HPD580 HPM620-60
Cy3™ HPF1310 HPX535-40 HPD565 HPM610-50
Cy5.5™ HPF1405 HPX645-40 HPD675 HPM710-50
Cy5™ HPF1390 HPX620-50 HPD655 HPM690-50
Cy7™ HPF1425 HPX710-50 HPD755 HPM800-70
DAPI HPF1205 HPX360-60 HPD400 HPM460-60
Dil(rs) HPF1315 HPX535-50 HPD565 HPM620-60
DiO HPF1250 HPX480-40 HPD505 HPM535-50
DsRed HPF1320 HPX545-30 HPD570 HPM620-60
Eosin HPF1270 HPX500-40 HPD525 HPM560-50
Ethidium Bromide HPF1350 HPX525-45 HPD565 HPM640-70
FITC HPF1250 HPX480-40 HPD505 HPM535-50
Fluo 3 HPF1250 HPX480-40 HPD505 HPM535-50
Fluo 4 HPF1250 HPX480-40 HPD505 HPM535-50
GFP HPF1240 HPX470-40 HPD495 HPM535-50
GFP(rs) HPF1250 HPX480-40 HPD505 HPM535-50
GFP(wt) HPF1225 HPX450-50 HPD480 HPM510-50
Hoechst 33342 & 33258 HPF1200 HPX350-60 HPD400 HPM460-60
LysoSensor™ Blue (pH 5) HPF1210 HPX380-40 HPD410 HPM460-60
MitoTracker Orange HPF1335 HPX525-45 HPD560 HPM620-75
MitoTracker Red HPF1340 HPX560-50 HPD590 HPM640-60
Nile Red HPF1360 HPX525-45 HPD565 HPM645-60
Oregon Green™ HPF1260 HPX485-30 HPD515 HPM545-30
Pacific Blue™ HPF1215 HPX400-30 HPD440 HPM470-30
PE (B,R) HPF1290 HPX525-45 HPD560 HPM585-40
Propidium Iodide HPF1370 HPX525-45 HPD565 HPM645-75
Resorufin HPF1375 HPX560-50 HPD595 HPM645-75
Rhodamine HPF1330 HPX570-20 HPD585 HPM620-60
Rhodamine Green HPF1235 HPX500-20 HPD515 HPM535-30
Texas Red HPF1375 HPX525-45 HPD590 HPM645-75
TRITC HPF1300 HPX525-45 HPD560 HPM600-60
YFP HPF1265 HPX500-20 HPD525 HPM550-40
YGFP HPF1235 HPX500-20 HPD515 HPM535-30

Superior Stabilife® Coating Technology

HPF™ fluorescence filters are manufactured using our patented Stabilife® coating technology. They are manufactured using two patented processes for the deposition of metal oxide thin film optical coatings: Reactive Ion Plating (RIP) and Hybrid Plasma Enhanced Deposition (HPED). Both processes yield highly dense, thin film coatings with extraordinary hardness, abrasion resistance, and adhesion to the substrate. Newport's Stabilife processes have been in full-scale production at our coating facility in Franklin, Massachusetts since the early 1990s.

Higher Film Density & Maximized Spectral Stability

For demanding applications such as fluorescence detection, wavelength stability is absolutely critical to insure dependable results. Newport's fluorescence filters with Stabilife coating technology have much higher spectral stability than competitors. One of the critical factor affecting the spectral stability of an optical coating is film density. Unlike competitor's unstabilized metal oxide thick film coating with less film density which typically contributes to environmentally induced spectral shift of 2-5%, Newport's Stabilife coatings have a much higher density and lower void ratio and are therefore less affected by water absorption. They typically exhibit total wet-to-dry shifts of less than 0.02% of wavelength.

Measured transmittance scans of a Stabilife filter at 0% and 100% relative humidity. Scans are exactly overlaid as no shift is discernable at the standard scan speed for a 30nm bandwidth filter.

Minimized Spectral Shifting for Temperature Variations

Measured temperature-induced wavelength shift of an unstabilized metal oxide ultranarrow bandpass filter compared to a Stabilife® ultra-narrow bandpass filter.

Stabilife coatings are typically 5 to 10 times less sensitive to thermal variation than un-stabilized metal oxide films as a result of film densification. Temperature change functions are a catalyst for moisture migration in thin films having a significant volume of voids. When un-stabilized films are exposed to high temperatures, moisture migrates out of film voids contributing to the wavelength change discussed earlier. The high film density and reduced permeability resulting from the Stabilife processes reduces this effect providing the maximum spectral stability available for all types of precision coatings including bandpass, dichroic, edge, notch and polarizer coatings.

Filter Holders

Newport's fluorescence filters are conveniently sized at 25.0 mm diameter, to be compatible with standard epifluorescence microscopy cubes or filter holders.  These filters are also compatible with standard one inch diameter Newport filter mounts.

Resources

Literature